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We additional showed that CPSI-1306, a small-molecule MIF inhibitor, inhibits the growth of TNBC cells in vitro. Mechanistic research unveiled that CPSI-1306 induces intrinsic apoptosis by alteration in mitochondrial membrane likely, cytochrome c (Cyt c) launch, and activation of various caspases. In addition, CPSI-1306 inhibits the activation of c